Ji-Won Lee

3 Questions 6 Answers 0 Followers

Questions related from Ji-Won Lee

Why my cell sorting yield too low ?

HI, I used FACS (BD Aria III) for cell sorting. I tested FACS cell sorting for in vivo cells isolation. I used Raw264.7 cells with stained anti-CD11b-PE and anti-CD45-APC. Cells were well...

24 September 2021 4,976 2 View

How extract native form of proteins from insoluble fraction (e.g insoluble pellet) ?

Recently, I try to extract the native form of proteins (for immunoprecipitation) from insoluble fraction (cell lysis pellet). How can I do? What kinds of lysis buffer are better? *Experimental...

13 March 2017 6,854 4 View

Why have my THP-1 cells differentiated by PMA during observation of inflammasome?

Hi all, I recently find the optimization of inflammasome studying conditions in THP-1 cells. THP-1 cells can be activated not only (non-PMA) LPS+ATP [Ref; invivogen] but also, after...

12 April 2015 9,715 2 View