Looking for collaboration possibilities.
I am curious. What is child cord blood?
Who are some key author to refer to?
18 May 2024 7,998 5 View
Dear Colleagues, We are conducting a meta-analysis to synthesize comparative studies investigating the relationship between proxies of sexual selection and species richness or speciation rate in...
07 April 2024 5,425 0 View
I have prepared two same materials with diffferent method and performed electrochemical testing. The electrode A which has higher specific capacitance gives higher (8 times) solution resistance...
25 March 2024 5,833 0 View
Hi, I am doing a pulldown in THP-1 wt lysate followed by MS-based proteomics. However I get a very low protein amount out of the cells (1 mg per 12 million cells). I use the following lysis...
14 February 2024 9,030 1 View
Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...
09 January 2024 2,970 4 View
Is the cif file for MXene(Ti3C2Tx) available? If yes, can anyone provide me with one for performing rietveld refinement? I have search from COD and AMCSD website and can't seem to find one.
13 November 2023 5,000 2 View
I'm wondering if you should differ between presence data of highly mobile species such as Raptors and immobile species (e.g. Plants). The dispersal of plants is limited to a certain distance, so...
23 May 2023 6,666 4 View
I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...
21 May 2023 9,628 3 View
I am attempting to use the Seurat FindAllMarkers function to validate markers for rice taken from the plantsSCRNA-db. I want to use the ROC test in order to get a good idea of how effective any of...
16 May 2023 2,909 1 View
15 May 2023 6,047 0 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 8,361 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
I am working on a project proposal (DFG project in Germany). I have the problem that I want to calculate how much funds I have to request for my test subjects (e.g., for qualitative interview of...
18 July 2024 1,690 0 View
Let n∈ℕ, where the function f:A⊆ℝ𝑛→ℝ, 𝐴 is Borel, and (A,d) is a completely seperable space. What is the name of the functions where the graph of f in any (n+1)-dimensional box of A×ℝ is...
17 July 2024 258 1 View
