There is a lot of published information about blood parameters in different teleost species but still isn´t a usefull tool for estimating fish health condition
1- Lack of fish-specific diagnostic kit for many substantial blood parameters such as protein and peptide hormones and ...
2- Hard procedure of blood sampling
3- Wide genetic variation due to high number of offspering per pair of fish
4- Lack of reference value for many diagnostic parameters in many fish species
5- Strong effect of environmental parameters on blood parameters, mainly because fishes are not homoiotherm and their metabolic rate and immune function is affected by temperature.
Syyed, thanks very much for taking the time to answer. I agree with you in all the external limitations.
I think a good start would be generating confiable blood parameters for "normal" fish. The IFCC suggests the use of minimum of 120 individuals for generating reference intervals. Obviously considering all the external factors that influence blood chemistry results in ectoterm fishes like salmonids, for eg.