Does anyone know which calibrator is the best for hyaluronic acid molecular weight estimation by conventional size exclusion chromatography ?

01 January 1970 0 142 Report

for Enzymatic biosynthesis and characterization of low dispersity hyaluronan oligomers i need to use calibrator. and i know SEC calibration based on non-HA standards like polyethylene oxide and pullulan led to approximately 2 and 10 times overestimation, respectively, when compared to HA-based calibration. for non-HA based calibration which calibrator is best?

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