Recently, I have been trying to clone human IgG and display it on surface of human B cell. Because the cell line Raji has IgM on it surface already, I need to have IgM gene knock out before It can be used. It seems very complicated.
Hi Shiqiang,
we have characterized >100 B cell clones (EBV-immortalized) and have some which do express low levels of only IgG or IgM or IgA - but there was no clone without any Ig-expression.
Thus, I would estimate that EBV-transformed lymphoid cell lines will most likely all behave like that.
Sorry, for having no proper line for you.
Best
Michael
Hi Michael,
I am sorry for the reply being so late.
Thank you so much for your information. In fact, I have tested some EBV immortalized B cells before and similar tresults were obtained. So now a pro-B cell line (JM1) has been used for the expression of recombinant BCR. JM1 just has no mIgs on its surface. But it seems that tranfection by lipofectin2000 and electroporation really did not work on this cell line. I am not trying to solve tranfection problem.
BTY, do you any experience on the transfection of B cell line and can share with us?
Thanks again.
Regards,
Shiqiang
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