I am trying to learn X! Tandem and would like to find a site where I can download mass spec data for analysis. Let me know if there is such a site out there. Thank you.
Hi everyone, Currently, I am trying to express and purify a peptide (insect peptide) using E. coli method. However, I have not yet made any progress. I am thinking about switching to insect cell...
01 February 2016 3,877 1 View
Hi everyone, I am trying to detect a very small protein (around 8 kda) and I found that my antibodies is not working (after repeated 4 times with various concentration) for this protein....
01 February 2015 1,946 11 View
I am trying to count the number of mosquito eggs. I am having a lot of trouble downloading imageJ for my mac. Do you have any tricks and suggestions will greatly appreciate. Thanks
01 February 2015 4,319 3 View
I have very inconsistent RNAi in vivo knockdown. I am looking for some alternative approaches. Hope I can use some of your expertise. Thank you.
09 October 2014 9,174 5 View
Hi, I am new to this topic. I constructed a recombinant protein with 6 His tag at the N-terminal. The expression host is BL21 (DE3)plysS. I checked the sequence multiple times and everything is in...
07 August 2014 2,750 21 View
“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...
11 August 2024 2,483 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View