Lewis Hun

6 Questions 21 Answers 0 Followers

Questions related from Lewis Hun

Does anyone know where I can download mass spec data?

I am trying to learn X! Tandem and would like to find a site where I can download mass spec data for analysis. Let me know if there is such a site out there. Thank you.

08 August 2016 9,413 0 View

What are the conditions for protein expression and purification with insect cell line?

Hi everyone, Currently, I am trying to express and purify a peptide (insect peptide) using E. coli method. However, I have not yet made any progress. I am thinking about switching to insect cell...

02 February 2016 3,857 1 View

What is a suitable software to count thousand number of eggs?

I am trying to count the number of mosquito eggs. I am having a lot of trouble downloading imageJ for my mac. Do you have any tricks and suggestions will greatly appreciate. Thanks

02 February 2015 4,295 3 View

What are techniques to detect protein without specific antibodies?

Hi everyone, I am trying to detect a very small protein (around 8 kda) and I found that my antibodies is not working (after repeated 4 times with various concentration) for this protein....

02 February 2015 1,920 11 View

What would be a good alternative approach for RNAi in vivo knockdown?

I have very inconsistent RNAi in vivo knockdown. I am looking for some alternative approaches. Hope I can use some of your expertise. Thank you.

10 October 2014 9,112 5 View

Does anyone have experience with Protein Expression and Purification?

Hi, I am new to this topic. I constructed a recombinant protein with 6 His tag at the N-terminal. The expression host is BL21 (DE3)plysS. I checked the sequence multiple times and everything is in...

08 August 2014 2,724 21 View