Are there any suppliers those provide thymosin beta 4 antibody that is suitable for ELISA?

We are planning to measure thymosin beta 4 expression in serum followed by tissue lysates using ELISA. We have looked into the R & D systems but they are not providing this ELISA kit. So, we...

11 December 2017 2,853 6 View

Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

We are trying to measure thymosin beta 4 protein expression in HUVEC cell line and in mouse muscle and brown adipose tissue using western blot technique. However, we are not getting any band or...

11 December 2017 8,969 4 View

What is the effective protease inhibitor to inhibit the non-specific proteases in case of Neutral endopeptidase (NEP) activity assay?

I am doing the neutral endopeptidase (NEP) activity assay in the pancreas and lungs by using fluorosent substrate (Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate) and NEP Specific inhibitor...

06 July 2014 2,233 2 View

How can we fix the "rpm or g" in centrifuge for any particular tissue sample after homogenization to separate supernatant from the pellet?

Most people in research usually use centrifuge to separate the supernatant from pellet but people do centrifuge at different rpm or g for different tissue samples. How do we usually fix the rpm or...

02 March 2014 4,067 4 View

What is the advantage of using 5 % milk in TBS-T as a blocking agent over 5 % BSA (Bovine Serum Albumin) in TBS-T

Which is the best blocking agent among BSA and Milk? Does it vary depending on the type of protein?

31 December 2013 1,021 35 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View