Ravinder Reddy Gaddam

6 Questions 16 Answers 0 Followers

Questions related from Ravinder Reddy Gaddam

Are there any suppliers those provide thymosin beta 4 antibody that is suitable for ELISA?

We are planning to measure thymosin beta 4 expression in serum followed by tissue lysates using ELISA. We have looked into the R & D systems but they are not providing this ELISA kit. So, we...

12 December 2017 2,908 6 View

Does anyone has any experience in measuring thymosin beta 4 protein expression using western blot?

We are trying to measure thymosin beta 4 protein expression in HUVEC cell line and in mouse muscle and brown adipose tissue using western blot technique. However, we are not getting any band or...

12 December 2017 9,039 4 View

What is the effective protease inhibitor to inhibit the non-specific proteases in case of Neutral endopeptidase (NEP) activity assay?

I am doing the neutral endopeptidase (NEP) activity assay in the pancreas and lungs by using fluorosent substrate (Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate) and NEP Specific inhibitor...

07 July 2014 2,313 2 View

Does anyone know the metabolic products of the substance P through neutral endopeptidase (NEP)?

Substance P undergoes metabolism to produce active biological peptide. Can anyone know the metabolic products peptide sequence of substance P through NEP.

07 July 2014 5,079 2 View

How can we fix the "rpm or g" in centrifuge for any particular tissue sample after homogenization to separate supernatant from the pellet?

Most people in research usually use centrifuge to separate the supernatant from pellet but people do centrifuge at different rpm or g for different tissue samples. How do we usually fix the rpm or...

03 March 2014 4,116 4 View

What is the advantage of using 5 % milk in TBS-T as a blocking agent over 5 % BSA (Bovine Serum Albumin) in TBS-T

Which is the best blocking agent among BSA and Milk? Does it vary depending on the type of protein?

01 January 2014 1,094 35 View