Why do you need know .." K+ concentration inside E.coli strain B ATCC 11303 under normal conditions"?

Please, let me know what do you mean: ".. under normal conditions"?

I would guess submillimolar. Of course depends on where it is grown. Guess You could calculate it approximately from average K+ gradient needed for particular organism to survive and K+ concentration of Your growth media...

Hi Ganesh,

Heldal et al 1985 AEM 50, 5, 1251-1257. Even though the authors did not specify the strain of E. coli they used, I assume that the date may be applicable for you. Please, have a look.

Some useful info possibly about K+ content in E coli could be found in:

Chang, C. F., H. Shuman, et al. (1986). "Electron probe analysis, X-ray mapping, and electron energy-loss spectroscopy of calcium, magnesium, and monovalent ions in log-phase and in dividing Escherichia coli B cells." Journal of Bacteriology 167(3): 935-939.

Best regards, Alexander.

Dear Paola Del Serrone,

Under normal conditions means overnight grown culture (~O.D 1.0) in L.B Broth at 37 degree Celsius.

- Ganesh