How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

Hi everyone, how can we remove the snow-covered areas in global scale?

We obtained the global results using remote sensing reanalysis dataset. But the snow-coverd areas should be removed (like the blank area in the picture below, sources: Purdy et al., 2016). Does...

20 September 2020 3,463 5 View

How to extract RNA from pancreas?

RNA extraction from pancreas of ruminants is very difficult due to the existence of abundant RNase. Previously, our research group tried to remove the pancreas from animal immediatley after...

20 September 2020 1,097 2 View

How to add a scale bar for images acquired from Molecular Device Imagexpress pico?

I saved some images when I scanned certain area using imagexpress pico. But don't know how to add scale bar either in system or image J. I am wondering if anybody can provide some help on that?

17 September 2020 8,068 4 View

How to get bending moment in Ansys shell181 element?

Hello, I would like to compare the bending moment of a tube with beam and shell element in ansys? The tube has the same dimension in beam and shell element. In beam element, i can get the...

24 August 2020 2,352 1 View

How should I design the N-terminal VC plasmid of BiFC assay?

I'm going to detect protein-protein interaction with BiFC assays and my target proteins are ER retention with KDEL motif. so I want to fuse VC to the N-terminal of my protein. My question is if...

22 July 2020 3,456 1 View

How to grow more BL21 auxothrophic strain cells?

Dear all, I have just purchased a BL21 auxothrophic strain (RF12) from Addgene. The cells come in the form of agar stab. I intend to grow more cells which will be ready to used for protein...

11 May 2020 8,384 3 View

Experience with immunostaining with anti-Hepatocyte Specific Antigen (Hep Par-1) antibodies in frozen tissue?

Dear scientists, I would like to stain the formaldehyde-fixed, OCT-embedded human liver tissues with anti-Hep Par-1 antibodies (DAKO; clone OCH1E5) for IHC staining. Do you by any chance having...

20 February 2020 3,017 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View