Does anyone know the best puromycin concentration to select human CD4+/Regulatory T cells transformed with lentivirus?

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Shijun Deng

Hi Sujit, i used 1.5ug/ml in HEK293 and 3 ug/ml in Huh7 cells. Not sure this information will help you. Good luck:)

Sujit Routray

Thank you, Shijun. I used 1.5 if/ml for 48 hours. Is the incubation period right according to you?

No problem. Yes, 48 hours for me. May be different in your case.

Great. Thank you for your valuable input, Shijun. Much appreciated.

Han Zhang

The puromycin concentration is totally different when using in different cell types. You need to optimize it within your cells.

Thank you, Han for your input. Yes, we did optimize it and found 2.5 ug/ml to the optimal. :)