Sujit Routray

2 Questions 6 Answers 0 Followers

Questions related from Sujit Routray

Does anyone know the best puromycin concentration to select human CD4+/Regulatory T cells transformed with lentivirus?

Concentration and the exact protocol, if anybody has it.

04 April 2017 3,078 6 View

Problem with CD4 proliferation using plate-bound anti-CD3?

Hi, I have tried culturing CD4 cells (isolated by Stemcell technologies) with plate-bound anti-CD3 (2 microgram/mL) with sterile PBS. Aspirating the PBS. Adding 200K cells/ well along with a...

01 January 1970 2,224 4 View