Does anyone know or have a reliable recombinant expression vector specifically for expressing and purifying Thioredoxin (Txn)? Preferably tagged?

22 March 2017 1 9K Report

I need to produce some Txn to use in an assay and it is becoming quite expensive. I found that E.coli Txn is more stable than human, and would like to look for an expression system. Thank you!

Mohammad Karimi

if you are looking for E coli Thioredoxin 1, its cloned in pET32 expression vector as solubility enhancer.

you can purify it according to below protocols:

http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps0607.html

http://www.currentprotocols.com/WileyCDA/CPUnit/refId-tx0704.html

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