I understand that just the canine tip is honed in bonobos and would like to verify that.
If so, could someone please give some insights on the IPTG concentration, the length of the induction period, the temperature during induction, and the cell line that was used. The gene is cloned...
15 August 2023 831 1 View
Hello, I want to silence my gene of interest using ptdT-RNAi vector, so what I need to know is either if the amplicon must include the 5’UTR region, the ATG or both. I know that the length has to...
30 May 2023 307 1 View
I've seen some publications ask for one year before posting elsewhere. A university librarian informed me that no such posting is allowed, on any type of site, including for members only.
20 November 2022 9,144 10 View
I collected a seaweed sample from the Kenyan coast (Mkomani). I was looking for Caulerpa macroalgae. I am uncertain of the specific name of the pictured seaweed. Can you help me identify the species?
20 October 2021 7,530 7 View
Hello, I am doing a microsatellite PCR and the bands appear with a few definition, the electrophoresis conditions are the following, TBE buffer 0.5X pH 8.4 (according to Sambrook, Molecular...
29 August 2021 2,860 3 View
I am monitoring the degradation of contaminants using immobilized enzymes on carbon support. However, to complete the mass balance of the degraded reactant, I have to perform a mass balance on...
06 April 2021 6,802 2 View
I am struggling on how to calculate this. I know I need the MW, but I am not sure how these all tie together.
01 April 2021 4,795 1 View
Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...
08 February 2021 10,086 3 View
Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...
06 January 2021 4,195 4 View
I was looking for Caulerpa lentillifera earlier today in Kenyan South Coas in Kibuyuni, Kwale county. I found a different kind of sea grape. I am certain that it is of the genus Botryocladia, but...
06 September 2020 1,147 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
When I stained the presynaptic sites of NMJs in the mouse lumbrical muscle, I observed significant background in my IFI. I fixed them with 0.5% formaldehyde overnight at room temperature....
16 June 2024 3,428 0 View