Does anyone know of a protocol for effectively isolating multipotent mesechymal stromal cells from mice adipose tissue?

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Tom Masi

There is a murine protocol (Chapter 3: "Isolation of murine adipose-derived stem cells" by Yu et al.) in Methods in Molecular Biology 702. Adipose-Derived Stem Cells: Methods and Protocols by Gimble and Bunnell (2011).

I use the protocol in Chapter 1 which is "Isolation and culture of rhesus adipose-derived stem cells" for harvesting/growing rat stem cells from adipose tissue. Works well for me.

Good luck!

Carolina Arruda de Faria

Thanks for the reference, but I wasn't able to access the book here from Brasil...