I wish to label a peptide in cells which corresponds to a well characterized protein, in order to track its fate. Could anyone suggest techniques that one could use to successfully do this besides fluorescent microscopy?
I want specifically edit the peptide at primary amine groups, preferably with fluorophores. Since the peptide target will be covalently attached to DNA, I will isolate the complex by ethanol precipitation. The fluorescence is to be quantified by slot blot or in 96 well format by a fluorescent plate reader.