Does anyone know of a good source for the pharmacological pathway of deschloroclozapine (DCZ)?

16 February 2023 0 10K Report

In particular, I'm wanting to know if DCZ is a metabolite of clozapine n-oxide or clozapine (differing and very little information in the literature), the enzymes that are involved in the metabolism and where this metabolism takes place in the body.

Badges
Science topic

More Lauren Nicole Beloate's questions See All

How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

How can I increase transfection efficiency in my DC2.4 cell line?

I have been transfecting a dendritic cell line for about a year now with about a 15-30% transfection efficiency that I would like to increase to 40-50%. I have used Neon Transfection...

13 February 2024 8,526 4 View

Users or Past Users of Peyote and/or San Pedro?

I am a senior anthropology student at California State University Northridge (CSUN). I am looking for a few people who use/have used peyote/San Pedro for their health and/or religious/spiritual...

20 November 2023 6,616 0 View

How to prevent capillary clogging for zebrafish microinjections?

Hi all, I've been working on a project that involves microinjecting cells into zebrafish. A big issue has been needle clogging. Once capillaries are loaded, cells clog the tip of the needle and...

17 November 2023 8,670 4 View

How to reduce zebrafish mortality post injection?

I am doing an experiment involving microinjections with zebrafish and have been experiencing high amounts of mortality. The embryos look healthy upon injection, but many of them are dying between...

17 November 2023 5,510 2 View

Is transformation of agrobacteria with freeze-thaw method possible with large plasmid?

I have a large plasmid (20kb) which I am trying to transform into chemically competent commercial EHA101 cells. I had success with transforming a 16kb plasmid into the same strain, but have been...

07 August 2023 8,527 6 View

Can anyone recommend the best way to culture the Salmon Head Kidney (SHK-1) cell line?

Hello, I'm very new to cell culture, so I am trying to determine the best way to culture my cells. I have been trying to culture salmon head kidney (SHK-1) since March and it is growing...

06 June 2023 5,362 1 View

Protocol for BriteVu Perfusion of Mouse Coronary Vasculature?

I am trying to use BriteVu casting reagent to perfuse the coronary vasculature and image using microCT. I have tried 1) perfusion via catheterization of the descending aorta with ligation of...

11 May 2023 2,589 1 View

What theoretical framework is suggested?

What theory would you suggest in a dissertation with the title of: 'Teachers’ Perceptions of Efficacy in Integrating Social-Emotional Learning into the Curriculum for Middle School Gifted Students' ?

06 March 2023 2,927 3 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Is it possible to quantify activity of the non-canonical Wnt signaling pathway via transcriptional readout genes??

Hello, From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These...

18 June 2024 4,699 0 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

Can someone help me getting the published version of a research article?

I needed help to get the published final version of a research article titled: "Alterations in placental long chain polyunsaturated fatty acid metabolism in human intrauterine growth...

11 June 2024 3,120 2 View

How to deal with product oscillations in the fungal chemostat?

I am operating a continuous cultivation at fixed carbon and nitrogen concentrations, and under carbon-limitation regime and varying dilution rates from 0.1 to 0.01/hr. Under these conditions all...

13 May 2024 2,510 0 View

Can you describe the process for initiating and titrating clozapine therapy in a patient with treatment-resistant schizophrenia?

Optimizing Clozapine Therapy: Overcoming Challenges and Enha... Can you describe the process for initiating and titrating clozapine therapy in a patient with treatment-resistant schizophrenia?

12 May 2024 6,010 2 View

How can a multidisciplinary healthcare team collaborate to optimize outcomes for patients receiving clozapine treatment?

Optimizing Clozapine Therapy: Overcoming Challenges and Enha... How can a multidisciplinary healthcare team collaborate to optimize outcomes for patients receiving clozapine treatment?

12 May 2024 1,159 0 View

What are some strategies for improving adherence to clozapine therapy in patients with psychiatric disorders?

Optimizing Clozapine Therapy: Overcoming Challenges and Enha... What are some strategies for improving adherence to clozapine therapy in patients with psychiatric disorders?

12 May 2024 9,707 0 View