08 July 2014 1 7K Report

I am looking for an antibody to detect human Galphai1/2 and Galphao for use in a GTPys pulldown assay. Does anyone know of a commerically avaliable antibody (other than Santa Cruz)?

More Kirstie A Bennett's questions See All
Is there any unpublished/published or ongoing works regarding secondary stroke prevention for young poeple living with stroke/TIA and their families?

I am working on a PhD proposal, which I hope to start in October 2024, focusing on non-surgical and non-pharmacological secondary prevention for young people living with stroke/TIA (YPLwS/T)...

23 March 2024 2,505 0 View

How to calculate effect sizes from multiple odds ratios for a single exposure variable for a meta-analysis?

For a meta-analysis, I'm including some studies that report the exposure variable in hours as categorical variables (e.g., "1 to 60 mins"; "61 mins to 120 mins"; "121 mins to 180 mins", etc.),...

19 April 2023 6,052 2 View

I am developing a cell and molecular biology laboratory class for upper level undergraduates. What lab manuals do you recommend (or not recommend)?

I would prefer one that is relatively recent and includes a section on gene editing i.e. CRISPR. Thanks much!

20 October 2022 2,589 0 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 6,278 3 View

What species (or genus even) of crab is this (bivalve using hermit type)?

Two weeks ago I found a peculiar crab in the intertidal zone in SW Florida. when found, the crab was not utilizing a shell. It was similar shaped to porcelain crabs found in our area, but with...

17 January 2021 2,009 4 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,917 6 View

Can anybody recommend a suitable and simple assay for measuring total salicylic acid content of foodstuffs?

Looking for a simple assay, i.e. colorimetric, to determine the SA content of food (if one exists). Most methods in literature are HPLC-based and ideally want to avoid this.

09 September 2020 9,243 3 View

How to Perform Feature Selection with Multilevel Encoded Categorical Variables?

I'm attempting to do feature selection on the variables on my dataset, but unfortunately the overwhelming majority of them are categorical. Currently they've all been encoded into binary...

29 July 2020 7,837 3 View

AlphaLISA SureFire assay troubleshooting - issues with phosphorylated protein detection?

We are currently working with a fluorescent AlphaLISA assay platform and are experiencing issues with detection of the phosphorylated protein of interest, despite numerous attempts at...

23 June 2020 7,301 2 View

What equipment do people use for larger scale (+ 1 L cell suspension) cell lysis for recombinant protein recovery?

I am currently working on a project where I need to recover and purify a number of His-tagged proteins for multiple 30 L fermentation runs. At smaller scales (around 1 L ferms) I have used a high...

25 February 2020 4,278 3 View

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Why activated CAR-Jurkat cell could not kill targets?

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