Does anyone know if it's possible to predict, from a genomic sequence, the gene structure ? And which software is available for download?

More Humberto Contreras-Cornejo's questions See All

What software do you recommend for analyzing dendritic architecture?

Hello everyone, I want to perform dendrite, sholl, and branch point analyses. I have seen ImageJ, Imaris, rebuild, and LasX. I have manually used all those software but have yet to have a good...

13 March 2023 5,658 2 View

Services for Proposal Writing? (for ESA, Eumetsat, Horizon 2020)?

Occasionally due to lack of time/people we need help writing proposals for research projects (Horizon 2020, European Space Agency, Eumetsat, etc). I would like to know if anyone knows of companies...

26 November 2022 7,230 1 View

What is the most significant expression of superior semicircular canal dehiscence that you have seen?

In those cases, what treatment decisions did they make? What variables did they consider?

15 February 2022 657 4 View

Anyone with experience in using the core set of ICF in vestibular rehabilitation?

I am working on a couple of papers with a core set for analysis of bilateral hypofunction and one case of Ramsey hunt III. I would like to share points of view on the use of the ICF

14 February 2022 6,326 0 View

How can the phylogenetic signal be measured or detected using mitochondrial DNA sequences?

I would like to know which software is the most commonly used or recommended for this analysis. Best regards, Cristian

29 March 2017 2,957 1 View

What's your experience treating bacteria (SA) with panton-valentine leukocidin ?

Are there any clinical guidelines for managing this kind of infection ?

22 December 2015 3,025 3 View

Does anyone know if it's possible to predict, from a genomic sequence, the gene structure ? And which software is available for download?

We have a genomic sequence and we want to identify a possible gene structure...Any expert? Or anyone doing this kind of work?

04 February 2015 3,927 2 View

Is anyone familiar with High Altitude Gliders?

Currently we have an ongoing research project to lift a small UAV (glider) to altitudes of about 100,000ft and let it glide back to a predetermined destination. The UAV would weight about 4kg,...

02 October 2014 928 7 View

Can somebody suggest me a protocol to determine glycosylation in yeast extracts?

I transformed a knockout yeast with a plasmid that contains a gen that complement the disrupted gene in the knockout yeast. They both are involved in glycosylation, so I want probe the...

20 March 2014 9,038 3 View

Erratic solution of the First (direct) Geodetic problem?

I attach the plot of a simulated flight (left) and of the mapping in lat/lon (left). In the simulation, the flight follows a path that only changes on the X-axis (straight line). All axii are in...

07 September 2013 375 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

Ravi Kant Upadhyay

You can use GeneMark™ - Free gene prediction software, and prediction of probable genes by Fourier analysis of genomic sequences si also possible. GeneScan can locate coding open reading frames and exonic regions in genomic sequences.

Harlan R Barker

Are you looking for a specific gene that you know exists in other species? If so then you can use orthologs of your suspected gene and the Exonerate program. The results of this will be superior to any de-novo prediction software if your case fits the requirements of the first sentence.

https://www.ebi.ac.uk/~guy/exonerate/