I want to understand the extraction efficiency of my compounds in the solvents.
Hi! What do you need ?
After PBMC isolation, we are not able to activate and expand T cells.
21 May 2024 6,124 2 View
Has anyone done extensive characterization of Innoprot immortalized corneal epithelial cells? Please cite the references if there are any!!! Thanks in advance !
16 April 2024 1,036 0 View
I am trying to grow a tissue on an insert under ALI on 24 well plate inserts and to see differentiation of the tissue I need to perform H and E staining , however given the smaller surface area of...
20 March 2024 4,764 0 View
I recorded the emission spectra of a compound in different solvents upon excitation at 500 nm. I observed emission peak at about 725 nm in water, thf and methanol with high emission intenisty. Is...
08 June 2023 4,912 0 View
There is standard of benzoic acid of 500ppm prepare in methanol: acetate buffer(55:45) during retention time in hplc there are two peaks what will be the reason for these two peaks
31 May 2023 2,199 1 View
I want to co-culture Neutrophils with leukemia cells (non-adherent) and want to separate the Neutrophils from the leukemia cells after the co-culture. If Neutrophils are purely adherent then I...
29 June 2022 1,153 2 View
Dear all, I am facing inconsistency between my DOS and band structures calculated from VASP. I have used the same algorithm IBRION = -1 and ISIF = 0, but ISMEAR = -5 for DOS and 0 for bands. From...
21 December 2021 8,064 7 View
What are the commands used for PVT variation analysis for CMOS circuit in LTspice?
25 September 2021 1,843 10 View
Dear VASP users, Suppose I add extra electrons in a system using NELECT in VASP and running scf calculations, then in the middle i am getting an error BRMIX: very serious problems the old and...
11 April 2021 2,671 1 View
How to calculate the RMSD between the docked pose of a ligand, and the same co-crystallized ligand?
22 March 2021 3,567 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View