I want to study the effects of PMA on cardiomyocytes (HL-1) cells. What is the working concentration of PMA to induce adequate response and how to block the action of PMA to see the effects.
if the purpose is to activate PKC then a more specific agent may be better. Once activated the action is pretty robust and last for several hours. Even if you remove the agent. Inhibiting the PKC will not stop the effect since downstream actuators are active.
You may try with the photo inducible agent that inhibit PKC, but I never tried it specifically.