How to dry nanoparticles without aggregating?

After freeze-drying my synthesized nanoparticles aggregate and do not disperse completely in water. Please help

05 October 2020 2,233 6 View

How to disperse freeze dried nanoparticles?

After freeze-drying, the nanoparticles could no longer be dispersed completely in water. Any suggestions on how to do it?

05 October 2020 7,814 4 View

Difference between systematic review and meta-analysis?

systematic review and meta-analysis

13 July 2020 4,041 18 View

Is there such a thing as glass, reusable tissue culture flasks?

We are looking for ways to reduce the amount of plastic waste in our lab.

10 December 2019 2,113 3 View

Please tell me easy Solvent Extraction Method for detection of metals in water samples ?

Solvent Extraction Method for detection of metals in water samples

20 December 2017 503 3 View

A.A i am a new researcher. please guide me about the p-type and n-type graph of band structure in wien2k

11 May 2017 5,317 5 View

If k.point optimization does not give the minimize energy and convergence graph.then what i can do?

for tetragonal structure

28 March 2017 4,244 3 View

What are the replication mechanisms for viruses?

hi everyone! what are the replication strategies used by viruses to replicate

10 January 2017 5,201 3 View

Protocol for native PAGE of catalase at differing pHs?

I'd like to establish how resistant a certain protein is to denaturing by running native PAGE gels of the protein incubated at different pHs. As a proof of concept, I'd like to run a gel of...

05 September 2016 8,398 3 View

Can anyone provide an alternate link for svmprot?

i want to use Svmprot for functional classification of proteins but the official link http://jing.cz3.nus.edu.sg/cgi-bin/svmprot.cgi is not working, can anyone suggest an alternate link that works...

16 December 2015 7,059 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View