If EtOH or Chloroform is used, what is the minimum concentration that can be used? I`d like to minimize the presence of those solvents on my final buffer for further purification step. Does anyone know of a good general rule of thumb for this?
The solvent conentration doesn't matter if you are going to form lipid micelles. You have to dry the lipid in a glass tube and sonicate the residue into your buffer of choice.