there are a lot of references available, e.g.:

Basic Clin Pharmacol Toxicol. 2019 Dec;125(6):490-498. doi: 10.1111/bcpt.13281. Epub 2019 Jul 19.Efflux transport of nicotine, cotinine and trans-3'-hydroxycotinine glucuronides by human hepatic transporters.

Järvinen E, Sjöstedt N, Koenderink JB, Kidron H, Finel M.

Methods Mol Biol. 2019;1981:55-73. doi: 10.1007/978-1-4939-9420-5_4.

Vesicle- and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function. Van Brantegem P, Deferm N, Qi B, De Vocht T, Annaert P.

Gert Fricker Thanks for the answers! Do you know if there are protocols where we can make these vesicles from animal tissue e.g. a liver sample from a mouse, rather than a cell line?

The publications of Peter Meier describing isolation of hepatic membrane vesicles may be helpful. e.g. J Cell Biol. 1984 Mar;98(3):991-1000.

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