Hi Naresh,

Since parylene contains aromatic rings, I would suggest plasma-mediated or UV-mediated hydroxylation. In these methods, in e.g. air atmosphere radicals such as -O*, -O*-H etc. are formed and that will lead to presence of -OH groups on parylene surface.

Having -OH groups is a good start to incorporate other chemical compounds onto hydroxylated parylene. I would suggest a book that is for me very helpful in that matter:

Greg T. Hermanson "Bioconjugate techniques'"

http://books.google.pl/books/about/Bioconjugate_Techniques.html?id=alooWMkRMk0C&redir_esc=y

Hello Naresh,

You can first immerse the parylene in serum to adsorb proteins onto the surface (eg. serum albumin, fibronectin, etc). You can then attach antibodies to the adsorbed proteins.

As Paulina mentioned you can also photo-oxidise the parylene surface with UV. This will create -COOH and -OH groups on the surface rendering it more hydrophilic.

Please have a look at the following 2 papers and let me know if you have any questions:

http://www.sciencedirect.com/science/article/pii/S0142961208010521

http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32662/abstract;jsessionid=405A3AF787A58AB3C11209B5A6239CCF.d02t04