We've bought alpha-thrombin (from sigma T1063) for apta-sensensor study that have a concentration of 1099 NHI units/mg protein. I'd appreciate If someone help to convert this value to molarity.
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/t6884pis.pdf
All depends on the thrombin specific activity. If it is pure, specific activity is expected to be of 2,500 to 3,000 NIH/mg.
Based on 2,500 NIH/mg your preparation at 1,099 NIH/mg protein is about 40% pure (or active). It contains actually about 0.4 mg active thrombin, which has a MW of 36,700 Da (for the alpha form). This makes a concentration which is approximately of 10 µM.
Mr. Amiral, thanks for your helpfull comment. I ll consider your proposal while preparing the solution.
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