I'm gonna design a project towards to prepare some nanopatterned collagen materials. Despite some reseachers perform this process with peeling off following the drop cast, i wanna use uv crosslinkable collagen to prepare the materials efficiently.
Collagen by itself is not UV-crosslinkable. You can however modify it such that it does become photo-crosslinkable. Have a look for instance at the following paper or search for other papers using GelMA:
http://www.ncbi.nlm.nih.gov/pubmed/26414409
For the actual photo crosslinking, you add a photo-initiator like irgacure or riboflavin (which actually works with blue light instead of UV). The photo-initiator forms the radicals which trigger the crosslinking of the GelMA
Dear Burak,
You can modify the collagen by introducing methacrylate group into the collagen. For the methacrylation, you can use methacrylic anhydride in the presence of base, such as DIPEA., or you can use methacrylic acid in the presence of NHS and EDC.
The methacrylated collagen can be crosslinked using UV light in the presence of photoinitiator.
You can read the following paper:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/
and
http://pubs.acs.org/doi/pdf/10.1021/bm0257412
Dear Farah,
Thank you so much for your valuable infos. I'll look at the papers.
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