Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

Same sample, significantly different trace for DMF vs. THF for cyclic voltammetry?

Dear reader, I've been having trouble with my cyclic voltammetry recently, but I then had a break through and managed to get apparently "clean" results using dry, deoxygenated THF and/or DMF,...

06 January 2021 4,312 3 View

Can I use GROMACS for field-theoretic simulation of copolymers?

I need to know if I can use GROMACS instead of LAMMPS for my undergraduate thesis wherein I will generate phase diagrams of copolymers using FTS. Thank you so much!

22 November 2020 3,725 3 View

How to quantify lncRNA/miRNA in ribosomal depleted RNA-seq?

I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...

11 November 2020 5,331 1 View

What are some essential steps when using THF for cyclic voltammetry?

I'm new to electrochem/cyclic voltammetry. I'm interested in measuring cyclic voltammograms of organic semiconductors in organic solvents, specifically, THF. I know that in principle I should be...

09 November 2020 2,298 4 View

At what stage of development do animals develop sentient?

I'm trying to find out in the development between being an embryo and an adult animal does the animal become phenomenally conscious? I'm especially looking for recommendations for articles on the...

30 August 2020 3,815 3 View

How to depolarize cultured primary neurons with KCl without them dying?

14 August 2020 1,488 8 View

Recommendations on model enveloped viruses that are easy and safe to propagate?

Hi everyone, We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact. In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to...

08 August 2020 6,731 4 View

Where can I purchase fluorescent silica nanoparticles?

Hi all, I want to purchase fluorescent silica nanoparticles (with and without a functional group to make it hydrophobic) in powder form. A variety of size options would be key. Preferably, if you...

04 August 2020 7,440 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View