i'm going to prepare liposome for protein functional essay, the protein i studied belong to Mycobacteria, could someone tell me which lipids I should use? are E.coli polar lipids feasible？
Zhendong Chen
Note that:
"... The most commonly employed phospholipid for the manufacture of nanoliposomes is lecithin (phosphatidylcholine), which is immiscible with water and is inexpen- sively isolated from egg yolk or soy. The composition of the phospholipid ingredi- ents and the preparation method of nanoliposomes determine if a single or multiple bilayers are formed. Fatty acids also make up nanoliposomes and their degree of saturation depends on the source. Animal sources provide more saturated fatty acids. These ingredients influence the phase transition temperature, which is the conversion from a gel to the more leaky liquid form, as explained in the next section. ...
... In order to produce stable vesicles in a reproducible manner, majority of nanolipo- some preparation procedures depend on the selection of right combination of phos- pholipid ingredients and their phase transition temperature (Tc). Generally, a pure phospholipid ingredient will not form vesicles at temperatures below the phase tran- sition temperature of the phospholipid molecule. However, this temperature require- ment is partially altered by the inclusion of cholesterol and other excipients as explained above [19, 20, 21]. With some preparation techniques, such as extrusion, microfluidization or homogenization, it is recommended that nanoliposome prepara- tion be carried out at temperatures above the Tc. This is in order to make sure that all the phospholipid ingredients are at the “gel state” and as a result have sufficient flexibility to align themselves in the bilayer structure of the nanoliposomes. ..."
See attached paper
Danaei M, Kalantari M, Raji M, Fekri HS, Saber R, Asnani GP, Mortazavi SM, Mozafari MR, Rasti B, Taheriazam A. Probing nanoliposomes using single particle analytical techniques: Effect of excipients, solvents, phase transition and zeta potential. Heliyon. 2018 Dec 1;4(12).
In general the best to study protein function is a liposome preparation containing all most rerpresentative phospholipids, i.e. unpurified lecithin, or low level purified lecithin
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