Need that calculation to obtain the degree of hydrolysis.
Hi, I am doing a pulldown in THP-1 wt lysate followed by MS-based proteomics. However I get a very low protein amount out of the cells (1 mg per 12 million cells). I use the following lysis...
14 February 2024 9,030 1 View
I am trying to simulate the combustion of black liquor solids from a soda process in Aspen Plus and the only information I have about the substance are ultimate analysis, organic/inorganic ratio...
09 August 2023 9,804 2 View
The restoration of blood volume in an excavated manner reduces hypoxia, necrosis and oxidative stress. The measurement of microcirculation in patients with acute pancreatitis could readjust the...
25 December 2021 901 9 View
I'm using the AbNova FISH probe for YES1 oncogene amplification (Catalog number FG0035) in H1975 cells. Since cell recovery prior to teh immunoFISH is perfomed in an eppendorf tube, I wouldn't...
30 June 2021 5,307 1 View
There seems to be a complex relationship between vitamin B12 and photosynthesis (prokaryotic and eukaryotic), but it is hard to pinpoint its meaning. - On the one hand, the pathways leading to...
15 August 2020 4,903 6 View
Hi, I would like to find out which KEGG pathways and/or gene ontologies (GO) are present in a microbial community from shotgun metagenomic data. I understand it can be done without pre-assembly...
23 July 2020 5,299 7 View
I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual...
26 May 2020 3,253 37 View
The anchovy is mostly intended for the production of fishmeal, because its taste and smell are not very pleasant before the taste of people, thus wasting the great nutritional value of the anchovy.
20 January 2020 3,596 1 View
If about 5-10 percet of gastric cancers have genetic bases, most of the guideliens does not include specific managemente for patients with familiar history of this disease. If those patients could...
28 December 2019 9,391 3 View
Recently, with the advent of potent antimicrobials and the use of imaging, the behavior of minimal esophageal peroforations fundamentally in the cervical region is treated medically or using endo...
03 July 2019 8,652 4 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
The adsorbate is Arsenic and the adsorbent is a soil
18 July 2024 332 8 View
Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...
17 July 2024 3,472 3 View
Aa
11 July 2024 4,645 3 View