How to do interdisciplinary research combining literature and visual arts?

I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual...

26 May 2020 2,889 37 View

What is the best method to store sectioned FFPE slides for long term storage?

I will be having FFPE blocks cut onto slides, I would like to know what would be the best method to store slides for long term storage. I don't want the slides to go bad. I am worried about...

16 January 2019 9,208 2 View

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28 September 2018 7,258 4 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

23 September 2018 4,882 3 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

08 September 2018 7,464 3 View

Can anyone recommend a sweetener that increases salivary secretion without disturbing original blood glucose levels?

To collect salivary samples (about 1 ml) from spider monkeys. The samples will be analyzed to determine cortisol, melatonin, serotonin and testosterone through ELISA tests. Thanks for any...

06 July 2018 3,605 3 View

Is it possible to detect serotonin levels in salivary samples through ELISA tests?

The technique is considered for a project to study physiological circadian patterns in free ranging spider monkeys, and thus the best suited sample source is saliva, because of its availability...

11 December 2017 5,978 2 View

Which ANTS/DPX concentration should I use to see solute leakage from liposomes?

I want to see how ANTS fluorescence in liposomes changes when adding triton.My idea is to encapsulate ANTS and its quencher DPX in the same liposome so that when adding triton fluorescence signal...

27 July 2017 9,640 1 View

How can I avoid nanoerythrosomes aggregation?

15 March 2017 10,022 6 View

Can orange/blue loading dye or green master mix can cause anomalous bands in my electrophoresis from a purified PCR product?

I am trying to purified a PCR product from gel extraction (2% agarose), for the purification I used PureLink Quick gel extraction and PCR purification combo kit (Invitrogen). And for the PCR...

28 September 2016 9,012 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

What is error #2070 in gaussian.how we do resolve it?

We want to ONIOM calculation in gaussian16. However, gaussian doesn't work. What should we do? Atom 15 layer R linked to atom 85 is also bonded to atom 161 in layer M bad...

15 February 2021 7,766 3 View