Let's say 1,6 and 1,5 respectively. I want to know how much RNA there is in the sample. Is there any equation to eliminate protein and phenol influence on RNA concentration calculated by NanoDrop?
According to Glasel, 1995 R(260/280) = 1.81 means that there is 40% nucleic acids and 60% protein in the sample. What does that mean? If the NanoDrop shows 260/260 = 1.81 and RNA concentration 100 ng/ul do I have actual concentrations of 40 ng/ul or 100 ng/ul and 150 ng/ul (60%) of proteins?
To obtain the amount of RNA without suppicious, you could measure it by fluorimetry. I use the Qubit from Invitrogen. It work well. But you will obtain "only" the quantification and not the contamination by protein, phenol or alcohol. Both nanodrop, fluorimetry, and gel electrophoresis are complementary.
I am aware of that. I use RNA 6000 Nano kit. I just wanted to get some more details about 260/280 and 260/230. Everything is written that over 1.8 it is good. I wanted to know more details.
If you want to be sure of the quantity of RNA, the best solution is to use a Qubit: http://www.lifetechnologies.com/us/en/home/brands/product-brand/qubit.html?s_kwcid=AL!3652!3!26240278226!b!!g!!+qubit&ef_id=jP1QEt5poKoAAAIU:20140321192348:s
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