I used

http://genepop.curtin.edu.au/

(local download version)

or

http://cmpg.unibe.ch/software/arlequin35/

Thank you very much! I used genepop for the calculation. Your suggestions were very helpfull!

Dear Anne

you can calculate Chi-square to test population equilibrium, by online calculator:

http://www.oege.org/software/hwe-mr-calc.shtml

or use the attached file.

Good Luck

Dear Koopmans,

You can use this web site to calculate the allele frequency and genotyping for 3 alleles

http://www.husdyr.kvl.dk/htm/kc/popgen/genetik/applets/kitest.htm

Hi there

http://scienceprimer.com/hardy-weinberg-equilibrium-calculator