I have been looking for clones 5D2 or MB20-11. We will be using these antibodies for research purposes.
You may use: anti-mouse CD20 (clone 5D2, isotype IgG2a, Genentech) or use : http://eu.ebioscience.com/mouse-cd20-antibody-purified-aisb12.htm
Anti-CD20 treatments. Mice were treated with one single dose of 50 or 100 μg mouse anti-mouse CD20 (clone 5D2, isotype IgG2a, Genentech) to evaluate depletion of endogenous or malignant B cells, respectively. For B cell transfer, splenic B cells were isolated by negative selection (Miltenyi Biotech), loaded with 10 μM SNARF or CellTracker Orange (Invitrogen), and injected intravenously (0.5 × 108 to 1 × 108 cells). Pretreatment with PTX (Sigma-Aldrich) was performed by incubating splenocytes isolated from GFP-expressing mice (3 × 107 cells/ml) at 37°C for 2 hours in RPMI containing 2% FCS and 200 ng/ml PTX. GFP– splenocytes isolated from WT mice were left untreated. Cells were transferred at a 1:1 ratio into Rag2–/– recipients. Clodronate was encapsulated in liposomes as described earlier and injected intravenously
Our first port of call for commercial antibodies tends to be antibodypedia, it is run by the Nature group and tends to give a good overview of what's out there.
You may use: anti-mouse CD20 (clone 5D2, isotype IgG2a, Genentech) or use : http://eu.ebioscience.com/mouse-cd20-antibody-purified-aisb12.htm
Anti-CD20 treatments. Mice were treated with one single dose of 50 or 100 μg mouse anti-mouse CD20 (clone 5D2, isotype IgG2a, Genentech) to evaluate depletion of endogenous or malignant B cells, respectively. For B cell transfer, splenic B cells were isolated by negative selection (Miltenyi Biotech), loaded with 10 μM SNARF or CellTracker Orange (Invitrogen), and injected intravenously (0.5 × 108 to 1 × 108 cells). Pretreatment with PTX (Sigma-Aldrich) was performed by incubating splenocytes isolated from GFP-expressing mice (3 × 107 cells/ml) at 37°C for 2 hours in RPMI containing 2% FCS and 200 ng/ml PTX. GFP– splenocytes isolated from WT mice were left untreated. Cells were transferred at a 1:1 ratio into Rag2–/– recipients. Clodronate was encapsulated in liposomes as described earlier and injected intravenously
http://www.biocompare.com/pfu/110447/soids/1593/Antibodies/CD20
please go through this link. Biocompare.com site. you will definitely find it useful for your search.
Regards,
Ajit
Thank you all for your answer. We really appreciate your suggestions.
Has anyone used the antibody (5D2) on a chondrosarcoma or osteosarcoma cell line?
Hi! You can request the anti-mouse CD20 mAb from genentech. You have to go to genentech web site and fill in a form online request in witch you will explain in detail your research project, and your in vivo protocol and how many mg of mAb you will need. You will be contacted by a sales representative who will send you a free batch.
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