I am looking for markers for some of the stem rust resistance genes "Sr11, Sr7b, Sr9b and Sr9e" in wheat.
I am trying to download EMMA 1.1.2, R package for windows, but I faced some problem. Did anyone try to do this and found that it works well?
09 October 2017 1,470 0 View
Hi All, If anyone used gel analyzer before, please help me. I want to know what is the accepted difference between the size of the bands. I mean, if I am looking to the 150 bp band, can I...
11 December 2015 8,219 4 View
Hi All, I am going to inoculate some wheat seeds with common bunt spores. I will use the dry method by mixing the wheat and spores together. I want to know the best time to do the inoculation. Is...
08 September 2015 901 2 View
Dear All, I am trying to analyze sequence data using DARwin software. The problem is that the data has missing values less than 20% and the program accept only data with less than 10% missing....
01 January 1970 3,373 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?
28 July 2024 4,886 2 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View