I want to detect which miRNAs are present in my EVs from mesenchymal stem cells. Would be possible to detect that without prior extraction of the RNA? If not, which methods do you recommend me for miRNA extraction?
Thank you in advance
Saurabh Mandal
Without either DNA or RNA isolation, few methodologies available and they seem costly. Just check these links.
Article Direct Quantification of mRNA and miRNA from Cell Lysates Us...
https://www.thermofisher.com/order/catalog/product/4391848#/4391848
Otherwise, various illumina based Next generation techniques, Nanostring based analysis, microarray platforms are available to detect all miRNAs present in a sample. you can go for that but u will need isolated RNA for analysis.
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