Does anyone know any methods for isolation of mitochondrial from rat nigrostriatal tissue in a sufficient amount?

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Citlalli Netzahualcoyotzi Piedra

My friend isolates enough mitochondrial fraction for oximetry essays just using the ventral horns of a really small segment of the spinal cord (rat), so, the following method should suits for your amount of tissue. Check: DOI: 10.1021/acschemneuro.6b00032

https://www.ncbi.nlm.nih.gov/pubmed/27090876

Farideh Iravanpour

Thanks a lot

Anna Stepanova

Farideh, check out this protocol

https://www.ncbi.nlm.nih.gov/pubmed/21913109

W. Brad Hubbard

I think it all depends on your exact definition of sufficient mitochondria. If you are talking about total mitochondria (synaptic and nonsynaptic), I have been able to get sufficient amount (at least 100 ug mitochondria) from around 30 mg of brain tissue.