How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

SiRNA transfection efficency and results interpretation ?

i am having a problem with the transfection of 2 siRNA , one for the longer isoform U and the other one of the sliced isoform S that is also the only transcribed. i am trasfection siRNA with a...

11 February 2021 8,145 3 View

Why do I see variability in mRNA expression in qPCR of the same gene (ubiquitin)?

Hi everyone! I use HaCaT cells and i transfect them with siRNA. After 48 h I extract proteins with Laemmli and RNA with trizol. I proceed with western blot to check proteins levels (and they are...

26 January 2021 5,738 3 View

Measuring small RNA abundance, without sequencing?

Dear all, Do you know any reliable and fast method to measure the levels of plant small RNAs (21,22 and 24 mer)? I am interested in only comparing the abundance of those small RNA between mock...

21 January 2021 4,002 6 View

What will be the life of siRNA (liquid form) at -20 degree, if kept unopened?

Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...

10 January 2021 6,346 6 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

Can I use FACS to optimize transfection by using a fluorescently labelled siRNA?

Dear all, I wish to find the optimal siRNA tranfection properties for my cell line. I have a fluorescently labelled siRNA which I plan to transfect by using different agents and quantify by using...

28 December 2020 5,349 7 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

How does one induce cell death using CRISP-Cas9?

Hello, I want to target a specific gene sequence using CRISPR-Cas9 to induce the death of the cells carrying that gene. I think that there are three basic approaches: 1.) Induce a frameshift...

20 November 2020 4,617 3 View

Western double band and si-transfection?

I kept getting double bands when western blotting, one at 25kDa and one at round 30kDa. I thought it could happen because I know that there are two isoforms from this gene. problem cames in here...

16 November 2020 1,283 3 View