Does anyone know an effective dicer enzyme to produce siRNA for homemade dsRNA ?

More Mehmet O Atli's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

07 July 2024 8,671 6 View

Reliable transfection method for Jurkat cells?

Hi to everyone! Does any of you have experience in transfecting siRNA in Jurkat cells and can provide a reliable method? I have both Neon transfection system and RNAiMAX Lipo, but to date none of...

30 June 2024 6,901 4 View

How to transfect siRNA into C2C12 Myotubes?

Can Opti-MEM without serum be used for transfection? Myotubes are typically at a high density upon successful differentiation. Does this reduce the efficiency of transfection?

15 May 2024 5,843 0 View

SiRNA services in India?

I am starting out on some siRNA based knockdowns on primary cells. Would anyone recommend good services they have used in India and any advice considering the advantage of modified and fluorophore...

09 May 2024 6,433 0 View

Why is RNP-RNA migrating faster than free RNA in EMSA gel at higher protein concentration?

Hello everyone, I have performed EMSA experiment with an RNA-binding protein and RNA. For both ssRNA and dsRNA, for higher concentration of protein, I observe a high intensity band at the dye...

18 April 2024 10,034 1 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View