Hi are you sure whether you do the protocol exactly or some other ...? CTAB method is one of the most useful methods in DNA extraction. i think low quantity is enough for your analysis, but be scrupulous about your diluting.
Hi Jalal. I need to know what exactly you are following in CTAB (send me ref of protocol or file if possible). What is your subsequent plan after extraction? I can suggest after knowing these. I am a Quaidian as well :).