I need a protocol to extract heme from hemoglobin.
ACETONE EXTRACTION OF HEME FROM MYOGLOBIN AND HEMOGLOBIN AT ACID PH., published in 1963 and fultext not accessible
This is the original paper for the process.
Teale, F. W. J., Cleavage of the hemoprotein link by acid methylethylketone. Biochim. Biophys. Acta 1959, 35, 543.
We do it regularly in our lab. So if you tell me what you need, I can have my person provide you the information.
The method in the paper that you have asked for is such that, you have to add 0.1N HCl to the Hemoglobin solution to acidify it to an extent it reaches pH 2.0, then add ice cold methylketone to it. shake it vigorously and keep on ice for 5 minutes and you will have aqueous fraction which has globin and ketone supernatant containing heme.
I have done this and it works properly. I just add equal volume of 0.1N HCl to hemoglobin solution and follow the same procedure as above.
Abhishek: Could you share with me a full protocol for it as well? My email is [email protected]
Dear Abhishek,
Would it be possible to send me the article frome Teale et al.? I am unable to download it from sciencedirect...
Thanks,
Arnaud
Has anyone tried to remove the heme from separate subunits alpha or beta of hemoglobin? Just curious what was observed. I did heme removal on hemoglobin using the acetone method and it worked perfect.
Hi Ishan Rathore, How you measure the heme from Aquas fraction finally after extraction? I want to measure heme from algae.....will this protocol work for algae too?
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