Does anyone know a method to specifically enrich nucleoli beyond isolating nuclei?

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What is currently the best model for the 30 nm chromatin fiber? I read about the tightly packed "solenoid", but is there experimental evidence for this model?

I'm trying to conceive a set of experiments to test the orientation of the nucleosomes relative to each other in a nucleosomal array that has assumed the 30 nm conformation. Any thoughts or...

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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No title

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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Pooneh Ardestani

I have never done that but I think Lamond lab (University of Dundee) is very good at that. you can use their protocols or contact them. http://www.lamondlab.com/f7nucleolarprotocol.htm

Kristopher Charles Hite

Thanks for the suggestion. I am working with plant cells which have the added hurdle of a cell wall in addition to the cell membrane. I wonder if I can modify this protocol to include grinding after liquid nitrogen flash freezing.