Does anyone know a lecithin molecule that can specifically binds to rhamnose?

21 March 2016 1 6K Report

I'm trying to express rhamnose and I need a way to detect its production through confocal microscopy and I saw in a meeting that some sugars can be detected by lecithin types. Does anyone could suggest a comercial one I could use, or some other way to detect intracellular rhamnose?

Adam B Shapiro

You mean lectin, not lecithin. A number of rhamnose-binding lectins have been described in the literature.

Here is a web page for a commercial source of one such lectin:

http://www.mybiosource.com/prods/Recombinant-Protein/L-rhamnose-binding-lectin-CSL3/datasheet.php?products_id=1120295

Badges
Science topic

More Otávio Bravim's questions See All

Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

26 May 2024 9,304 3 View

Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

26 April 2024 8,440 3 View

False positive in yeast transformation?

Recently I performed an yeast transformation and I got a few colonies in the selective plate. The strain is very modified, with four auxotrophic markers (CRISPR) and we are struggling to insert...

23 August 2023 2,703 2 View

Have you done or thought about doing an article about the impact of covid 19 in your research / work area? If so, which one?

27 December 2020 3,973 12 View

Error in Abaqus: elements have zero transvere shear stiffness. How I solve this problem?

I'm modeling a one-dimensional problem of an axially loaded crimped beam in an elasto-damage regime, which is the damage model is that of Marigo. To add the damage I used a UMAT subroutine. Create...

13 December 2018 3,477 4 View

What is your biosynthetic cornea project current stage?

Is there any other material than PMMA that actually works? Best Regards

12 February 2018 4,755 1 View

Does anyone could suggest a Acetyl-CoA carboxylase assay in/for Saccharomyces?

I have transformant Saccharomyces, but I'm having trouble to find a good assay for detection of the ACC activity. Does anyone could suggest one?

29 June 2016 7,561 2 View

Can anyone suggest a protocol for protoplast transformation for the transformation of Bacillus subtilis wild?

I have wild strains of Bacillus, and I'm with real problems in transforming them... I would like to try a protoplast transformation, can anybody suggest a optimized protocol??

02 August 2015 8,139 4 View

Does anyone have a Heat shock protocol for Bacillus subtilis H20.1, MT800 and 1012 and competent cells?

I'm having trouble to transform Bacillus subtilis in my lab, I tried some electroporation protocols, but they are not working. So I wonder if anyone would have a heat shock protocol I could use....

05 July 2015 7,754 2 View

What are the best sources for Military Veterinary Medicine?

Are there many theoretical sources and practical experiences published in the field of Military Veterinary Medicine? Where are they? Can anyone provide any literature of references?

14 January 2013 7,832 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View