Does anyone know a good system for hydroponic culture of Arabidopsis (not sterile culture) that does not lead to hypoxia.

More Janusz Niedojadło's questions See All

How to remove the preprint of the paper from the ResearchGate?

How to remove the preprint of the paper from the ResearchGate? The paper cannot be submitted to journals because they find the similarity to this text. I would like permanently remove this...

30 June 2023 5,506 4 View

Do you know of any company that sells pipette tips that are resistant to 70%-98% concentrated sulfuric acid?

Dear RG Community, Do you know of any company that sells pipette tips that are resistant to 70%-98% concentrated sulfuric acid? I am familiar with Chemical Resistance Charts. Standard...

30 August 2022 3,970 0 View

What are the limits of ACTIVE flight of various animals - Maximum Altitude (km) and Longest Continuous Travel Distance (km)?

Dear RG community, I am looking at the limits of ACTIVE flight of various animals. I am only interested in animals that are capable of active flight. Active flight (also called powered flight)...

28 May 2020 5,638 3 View

DIN 22252:2012 - actual or obsolete standard?

Got different information according to standard DIN 22252:2012 "Round steel link chains for use in continuous conveyors and winning equipment in mining". Is it outdated or current? I do not know...

14 January 2020 4,588 0 View

How to perform surface EMG recordings for diaphragm? Where the electrodes should be placed?

Numerous studies using sEMG to recorded diaphragm activity was performed in a various methodology. For example, in one of the study to record the activity of the diaphragm, the electrodes were...

08 May 2016 9,250 3 View

IF on endomembranes and vesicles. Is there a better method than saponin in PBS?

I am setting up a protocol for IF on endomembranes in hippocampal neurons. Thus far, it has been difficult to obtain crisp clear image even when using saponin permeabilization, as it seems that...

11 February 2016 5,831 7 View

How to assess diaphragm strength in spirometry? If anyone knows the literature where you can find the information about this?

Diaphragm assessment in spirometry. I would like to evaluate the effectiveness of breathing exercises on function and action of the diaphragm.

11 February 2016 673 5 View

Fourier transform of AFM images - what does FTT show?

Got a lot of ideas and/or information according what FT of AFM/MFM images shows. For instance: the directionality of magnetic field, elongation of domains, etc. According to another scientist it...

18 November 2015 2,722 7 View

Does anyone know a good article/review that compares all demyelination models used in science?

Hello, I am currently looking for new insights which give me a better and wider understanding of de- and remyelination processes. I would like to have some statements about pros and cons of...

02 March 2015 8,724 3 View

How to correctly calculate Normalized Google Distance?

I’m trying to implement semantic similarity based on Normalized Google Distance and i have many problems to obtain correct data. I tried to simulate browser, get data via Google API (PHP, Java, R,...

01 February 2015 2,955 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View