Does anyone know a good protocol to extract glycosphingolipids from cell membranes to be than anlysed by TLC?

More Francesco Renzi's questions See All

How to determine the charge passing during a cyclic voltammetry?

Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...

29 July 2024 3,733 5 View

Differences between Surface Tension and Interfacial Tension in Liquid-Gas Phases?

Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...

18 July 2024 382 5 View

EIS spectra analysis software?

Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...

10 July 2024 8,880 4 View

How to perform a double step optimization with ORCA?

Hello to everyone, I am trying to launch a BLYP optimization with ORCA starting from an already converged calculation at BP level ,exploiting its orbitals and geometry as a guess. I tried...

24 June 2024 3,768 2 View

Is a binary classifier based on Gaussian models resistant to the problem of training set imbalance?

A binary classifier based on multivariate Gaussian models, which estimates the mean vector and the variance-covariance matrix during the training phase and returns the class with the highest...

23 June 2024 10,114 1 View

Odd structures in transmission electron microscopy?

Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco

09 May 2024 2,070 11 View

APOe isoforms Sanger sequencing - good primers and protocol?

Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...

24 April 2024 6,359 4 View

Why do we use an oblique rotation in Factor Analysis when data is highly correlated?

What should we do with high-correlation data? Did we remove them, or do they not matter? WHY?

12 April 2024 9,429 3 View

Double-counting of emissions in the context of ISO 14068 - Carbon Neutrality assessement?

Greetings. In the context of the new ISO 14068 on Carbon Neutrality, how is double-counting of emissions between two subjects been dealt with? Suppose Company B is assessing the carbon neutrality...

03 April 2024 3,596 1 View

Do age- and/or sex-specific allometric scaling indexes for muscle mass on body weight exist?

Is there any standardised index to estimate, more or less precisely, muscle mass from body weight (e.g., Bodyweight * index)? Any reading suggestions are much appreciated.

27 February 2024 8,285 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Richard S Brush

We do this routinely in our lab. Check out this paper:

Retinal sphingolipids and their very-long-chain fatty acid-containing species.

Invest Ophthalmol Vis Sci. 2010 Sep;51(9):4422-31. Epub 2010 Apr 14

Francesco Renzi

Thnak you very much for your help!!

Salman B. Hosain

We do it often. You can try this paper too.

Glucosylceramide synthase upregulates MDR1 expression in the regulation of cancer drug resistance through cSrc and beta-catenin signaling.

You can try this paper too.