Xiaoqiu, I am not sure if you want to do tissue culture of cells enriched for endometrial stroma or epithelial cells or are looking for the purposes of analyzing the cells at the time of necropsy. In the former case we isolate the cells for tissue culture using mechanical dispersion, gentle emzymatic tissue digestion (librase), and then filtration separation (two step filtration using 100 and 40 um filters). The stromal cells are collected in the final filtrate whereas the epithelial cells are retained on the filter (40 um filter). Alternatively, if you are interested in studying gene expression in the different cellular compartments of the endometrium then you are stuck with using laser capture microdissection.

Thank you very much, Dr. Forster. I am going to analyzing the cells at the time of necropsy. So I guess I have no choice but laser capture.