28 April 2014 1 3K Report

We want to separate fibroblasts and schwann cells (with p75 marker).

More Matthias Krienke's questions See All
Can I synthesize my own primers for indexed Illumina library preparation?

Dear experts, I am planning to generate a large numbers of Illumina libraries in an ongoing project. These will be amplified via a single PCR step, in which the indexed adapters are added to the...

12 May 2024 8,656 1 View

Do you know any (recent) examples of System Dynamics used in sociology?

Most SD research seems to happen in adjacent fields such as economics or geography. I struggle to find papers that make use of this method for core topics of sociology. There seems to be more...

29 January 2024 2,171 1 View

How can the issue of population be treated and handled in an empirical research study?

I suppose, first and foremost, that the total physical number of the organisations to be studied should be ascertained. If the total number is 30 and above which is statistically large, a sample...

01 August 2023 8,596 3 View

How can I unde the accidentally deletion of entries in Bionumerics ? Is there an undo function ?

How can I unde the accidentally deletion of entries in bionumerics ? Is there an undo function ?

20 July 2023 9,770 0 View

What causes bubbles to form in COP polymer slides under heat treatment?

I'm trying to manufacture microfluidics into 75x25x1mm COP polymer slides. The process is 11 min long and using 150°C (COP Tg=100°C) temperature around 20-30bar pressure. Problem is during the...

09 July 2023 4,908 3 View

Do Orginators of Sample Size Determination formula deserve credits?

Michael Slovin in 1960 (cited in Guilford & Frucher, 1973) pioneered the sample size determination formula thus: N/1+Ne2. He received no credit for this pioneering efforts. Seven years later...

19 April 2023 8,185 9 View

What does my outcome from "Plotting residuals vs. fitted values of a binomial GLMs" mean (in R)??

For my work i did some binomial GLMs, where i compared locations with the presence of a species (wolves) against pseudo absence. Now i wanted to evaluate my Models by plotting the residuals of...

10 November 2022 6,486 3 View

How to coalculate the Area-Size of a Polygon-Vector-Layer within another in QGIS?

I created Buffers (shapefile) around several Points of Interest. Now i want to calculate how much of these Buffers-Areas overlap with other Polygon-Shapefiles (e.g. Protected Areas, Military...

09 October 2022 4,273 4 View

PyMol: Solid line between two residues. What is it?

Hi! I am stumbling over a weird thing which PyMol shows me in some structures: The attached image contains one of them. Between two residues from different chains, I'm getting a solid line, like a...

04 September 2022 5,116 2 View

Does it make sense to change redundant items from a questionnaire when the construct has too high composite reliability and cronbachs alpha?

For my thesis I'm trying to find out what influences people in their intention to use medical video consultations. I'm using an adopted UTAUT2-based model. With the first 100 responds I did a...

23 May 2022 3,643 7 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close