Hey, I've worked with S. aureus a fair bit for protein pulls, and it's a pain because of that thick cell wall, but a solid go-to lysis buffer I've used is a Tris-based one: mix up 50 mM Tris-HCl at pH 7.5-8.0, throw in 150 mM NaCl, 1-5 mM EDTA, and 0.5-1% Triton X-100 or NP-40 for the detergent kick. To really crack it open, add lysozyme at 1 mg/ml and if you can swing it, lysostaphin at 20-50 U/ml—incubate the cells in that for 30-60 minutes at 37°C, then hit it with sonication or bead beating for a few cycles. Don't forget protease inhibitors like PMSF or a cocktail to keep things stable, especially if you're dealing with DUB stuff; I've found this setup doesn't mess with most inhibitors, but test it out since some reducing agents might interfere if your protocol calls for them later. If you're looking for something off-the-shelf, the B-PER reagent from Thermo works decently too, just spike it with extra enzymes for Gram-positives like aureus.
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