Does anyone know a good antibody against EVA1A (also called TMEM166 or FAM176A) for western-blot and/or IF and/or IHC ?

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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Issues with Permeabilization during Flow Cytometry?

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Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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How does an antibody have full intact antibody + half antibody?

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Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

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RIA. How to measure?

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Anyone know of a good progesterone antibody?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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25 February 2021 8,404 3 View

Konstantin Yakimchuk

Hello,

You may check a free antibody database provided by www.labome.com. There are several antibodies against EVA1A (TMEM166), suitable for Western blot, IF and IHC. For instance, Invitrogen provides two anti-EVA1A antibodies, which can be used for both Western blot and IHC (reactivity with human, mouse and rat tissues): http://www.labome.com/gene/human/EVA1A-antibody.html. In addition, Santa Cruz provides several goat polyclonal EVA1A antibodies also useful for both Western blot and IHC. Also, Life Span Biosciences and Abnova have antibodies to human EVA1A suitable for Western blot. Hope this comment will help.

Bacquin Agathe

Thank you!

Always difficult to choose between different antibodies for the same protein, without knowing if it really works (no publication available).