Can you recommend a published study or textbook that can explain how to extrapolate in invitro concentration to mice dose and vice versa?

I need to conduct a preliminary in vitro study on different types of oligosaccharides fermented by bacterial culture. I need to translate the concentration of the oligosaccharides with highest...

04 May 2018 9,230 1 View

Does anyone know a laboratory in asia where I can send mice fecal samples for microbiome profiling?

My study will require microbiota profile. Unfortunately, we dont have facilities and skills to do that or collaborators. So im thinking of sending the samples for analysis.

31 December 2017 568 4 View

Hello everyone. Does anyone know a laboratory that offers cyanidin 3 glucoside analysis?

I have some rice samples that needs to be analyzed for specific anthocyanins such as cyanidin 3 glucoside. Thank you.

09 October 2017 6,701 1 View

Hello everyone. Does anyone know a laboratory where I can get my samples analyzed for cyanidin 3 glucoside?

I need a specific anthocyanin analysis for my rice samples.

09 October 2017 1,695 2 View

How do you compute for sample size in a rat experiment with 4 groups?

I am using two powered means in stata to compute for sample size coz the only study I can find that is similar to mine has only 2 groups. But I will be using 4 groups in my study. How can I...

09 October 2017 9,870 10 View

Can you translate convert in vitro enzyme inhibition into in vivo dose in mice?

I will be doing an invitro study on lipase inhibition of an extract vs. orlistat using IC50. I will be using different concentrations of the extract to identify the concentration that is...

09 October 2017 4,754 3 View

Are elisa assay kits with 96 plates need to be used all at once? or can it be used in before and after studies with month-long intervals?

I will be doing a study in rats and I will purchase a 96 well assay for insulin. I will use only 30 rats and measure it twice.

09 October 2017 6,084 3 View

Anyone knows a laboratory in Asia or the in US where I can get my samples analyzed for piceatannol contents?

Also, around how much would it cost?

03 April 2017 1,461 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View