Does anyone have tips for RNA-seq from FFPE?

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If I state KPOINT = 1 in VASP, does this mean I lose periodicity?

Hey everyone, my research is related to computational materials field and DFT usage. my professor told me that if I'm putting Kx for example as 1 (Gamma division), it means the system ignores the...

23 December 2020 4,227 5 View

Where can I find 5G/4G cellular network data set with parameters like Received Signal Strength (RSS), Channel State Information (CSI) of Multiple UEs?

I need a dataset for authenticating genuine user from spoofer based on physical attributes like RSS, CSI using Machine Learning. Since i dont have access to physical hardware Software defined...

13 July 2020 6,321 4 View

Oral gavage with methylcellulose?

I'm planning an in-vivo experiment with a drug dissolve in 0.5% methylcellulose and 0.2% tween 80. Which methyl cellulose should I order? The viscosity seems to change between products ( measured...

24 August 2019 1,604 2 View

Do physicians provide lower quality of care to close relatives or friends?

It is against the law in some states to provide care to close family members and many codes of physician ethics contain guidelines not to do so. Does anyone know of any empirical evidence...

19 October 2016 6,117 6 View

Is there a phenomenon of "elderly lies"?

I recently spoke to a group of seniors and was confronted with a simple question that I've never thought about before. See my fuller account of the event in the link. What is an elderly lie,...

30 May 2016 5,015 3 View

Can you recommend an instrument for mesuring attitudes about reading in children age 6-10?

Looking fro something that is relatively quick and easy to administer as a pre and post intervention measure.

09 April 2016 6,746 1 View

Did anyone try the Mk 1 MinION?

I want to know if it is working as advertised. How many reads am I to expect in realistic settings? How bad is the quality?

07 January 2016 2,058 1 View

Is there a preferred way to use projection mapping for the world?

I am doing a research paper on cartography. I know that there are different opinions regarding the most useful type of projection map. I'm just looking for some opinions and why you think, for...

22 September 2015 8,217 14 View

What are the best ways to content-analyze social media streams?

I'm looking for recent developments in automated analysis of Twitter, Facebook, or any other text-based social media streams. What are researchers able to extract? How are facts gathered,...

01 June 2015 9,397 9 View

How do you determine whether a news source is reputable? Or a news story is reliable?

We are thinking through some of the problems in distinguishing reputable news from phony ones. What might give you a clue that an online story you are reading is bogus, fake, or unreliable? We'd...

20 May 2015 6,521 41 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View